Human Anti-SIRPA Recombinant Antibody (PABJ-0084)
CAT#: PABJ-0084
This product is a Human antibody that recognizes Human SIRPA. This antibody is specific for human and cynomolgus monkey SIRPα. PABJ-0084 potently antagonizes the SIRPα/CD47 interaction.
Tested Data
Gene Expression
ELISA
Figure 1 Human Anti-SIRPA Antibody (PABJ-0084) in ELISA
ELISA analysis of PABJ-0084 was performed by coating with Recombinant Human SIRPA.
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 2 Human Anti-SIRPA Antibody (PABJ-0084) in WB
Western blot analysis of PABJ-0084 was performed by loading Recombinant Human SIRPA.
PABJ-0084 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane1: Reducing antigen (0.1 μg)
Lane2: Reducing antigen (0.3 μg)
Lane3: Reducing antigen (0.6 μg)
DB
Figure 3 Human Anti-SIRPA Antibody (PABJ-0084) in DB
Dot blot analysis of PABJ-0084 was performed by loading Recombinant Human SIRPA.
PABJ-0084 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Normal Tissue
Figure 1 Cerebral cortex
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_7_3.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (fHDF/TERT166, RPTEC TERT1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198053-SIRPA
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human SIRPA
- Species Reactivity
- Human, Monkey
- Applications
- ELISA, Block
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Blocking.
Target
- Alternative Names
- BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; CD172 antigen-like family member A; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; inhibitory receptor SHPS-1; macrophage fusion receptor; myd-1 antigen; tyrosine phosphatase SHP substrate 1
- Gene ID
- 140885
- UniProt ID
- P78324
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Exceptional Tool for Immunotherapy Research
I'm thrilled with the performance of this antibody in our immunotherapy research. Its ability to block the SIRPA/CD47 interaction has provided us unique insights into tumor immuno-evasion mechanisms. This has been instrumental in designing experiments that explore innovative cancer therapies.
Reliable and Stable Reagent
This antibody is an excellent addition to our lab. Its stability, even after multiple uses, has been impressive, providing consistent results in our assays. It's become a staple in our studies involving SIRPA, ensuring reliable data in every experiment.
High Specificity and Sensitivity
We've used this antibody extensively in our ELISA setups, and its specificity and sensitivity are truly remarkable. It has significantly improved the precision of our measurements, giving us the confidence to make data-driven decisions in our research projects.
Q&As
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What is the primary application of the Human Anti-SIRPA Recombinant Antibody?
A: The Human Anti-SIRPA Recombinant Antibody is predominantly used for blocking the SIRPA/CD47 interaction. This function is crucial in studying immune responses and can be applied in experiments focusing on immune checkpoint blockade, aiding cancer research by potentially unleashing anti-tumor activity.
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What specific targets does this antibody bind to?
A: The antibody specifically binds to SIRPα, a protein expressed on myeloid cells, recognizing both human and cynomolgus monkey proteins. This specificity allows for precise studies involving these target organisms, making it useful for preclinical models.
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What diseases can be studied using the Human Anti-SIRPA Recombinant Antibody?
A: Beyond cancer, this antibody can be employed in studies of various immune-related diseases, including autoimmune disorders and conditions involving immune suppression. It helps in dissecting the pathways involved in immune evasion and tolerance.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0084, RRID: AB_3111604)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| HPAB-0024-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0025-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5); Fab Fragment | ELISA | Humanized Fab |
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